Cell division in large embryos is coordinated by spatial waves of Cyclin B–Cdk1 activity that spread through the cytoplasm and affect cortical contractility. However, ...
High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL CA-360), 1% DOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8) Low ...
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